On day 1, pre-treatment X-rays showed an infection deep within the underlying bone, tissue, and around the nail, with signs of initial biofilm formation. After three days of R327G treatment, the wound dried up with the infection clearing, and the toe responded well to treatment. On day 7, the wound completely dried up, with no signs of biofilm surrounding the toenail, and swelling had significantly reduced. Surgical intervention was averted, such as limb amputation, which is common in patients with diabetes.